Novel deaminoneuraminidase and process for producing the same

C - Chemistry – Metallurgy – 12 – N

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C12N 9/24 (2006.01) C12N 1/20 (2006.01) C12P 19/00 (2006.01)

Patent

CA 2193500

A deaminoneuraminidase is harvested from the product of culture of a sphingobacterium such as Sphingobacterium mOL12-4s. This enzyme acts on a simple or complex carbohydrate containing deaminoneuraminic acid so as to hydrolyze the ketoside linkage of the acid and form free deaminoneuraminic acid and a simple or complex carbohydrate completely or partly deprived of deaminoneuraminic acid, but does not hydrolyze the ketoside linkage of N- acetylneuraminic acid or N-glycolylneuraminic acid of a simple or complex carbohydrate containing the N-acylneuraminic acid.

La déaminoneuraminidase de la présente invention est récoltée du produit issu de la culture d'un sphingobactère tel que Sphingobacterium m0L12-4s. Cette enzyme agit sur un glucide simple ou complexe contenant de l'acide déaminoneuraminique permettant l'hydrolyse de la liaison cétoside de l'acide et la formation de l'acide déaminoneuraminique libre ainsi qu'un glucide simple ou complexe totalement ou partiellement privé d'acide déamnoneuraminique. Toutefois, cette enzyme n'hydrolyse pas la liaison cétoside de l'acide N-acétylneuraminique ou de l'acide N-glycolylneuraminique d'un glucide simple ou complexe contenant l'acide acylneuraminique.

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