C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12N 15/66 (2006.01) C12N 15/79 (2006.01) C12N 15/90 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2759882
The invention refers to a novel method for cloning DNA molecules using a homologous recombination mechanism between at least two DNA molecules comprising: a) providing a host cell capable of performing homologous recombination, b) contacting in said host cell a first DNA molecule which is capable of being replicated in said host cell with a second DNA molecule comprising at least two regions of sequence homology to regions on the first DNA molecule, under conditions which favour homologous recombination between said first and second DNA molecules and c) selecting a host cell in which homologous recombination between said first and second DNA molecules has occurred. In particular, it relies on the use of the E. coli RecE and RecT proteins, the bacteriophage Red-alpha and Red-beta proteins, or the phage P22 recombination system. The beneficial effects of concomitant expression of the RecBC inhibitor genes (e.g. Red-Gamma) is also examplified.
Buchholz Frank
Stewart Francis
Zhang Youming
Europaisches Laboratorium Fur Molekularbiologie
Norton Rose Canada S.e.n.c.r.l.,s.r.l./llp
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