Novel dna polymerase

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/54 (2006.01) C12N 9/12 (2006.01)

Patent

CA 2241170

A DNA polymerase having the following characteristics: (1) showing a polymerase activity on a complex employed as a substrate wherein a primer has been annealed with a single-stranded template DNA higher than the activity on an activated DNA employed as a substrate; (2) having a 3'5' exonuclease activity; and (3) when subjected to a PCR reaction with the use of a .lambda.- DNA as a template, being capable of amplifying a DNA fragment of about 20 kbp without the necessity for adding any other enzyme; a protein constituting the DNA polymerase; a DNA containing a base sequence encoding the same; and a process for producing the DNA polymerase. This DNA polymerase is a novel one having both of a high primer extensibility and the 3'5' exonuclease activity.

ADN polymérase ayant les caractéristiques suivantes: (1) elle présente une activité polymérase, sur un complexe employé comme substrat dans lequel une amorce a été renaturée avec une matrice d'ADN à simple brin, supérieure à son activité sur un ADN activé employé comme substrat; (2) elle a une activité 3'?5' exonucléase; (3) quand elle est soumise à une PCR avec utilisation d'un ADN .lambda. comme matrice, elle est capable d'amplifier un fragment d'ADN d'environ 20 kb sans nécessiter l'adjonction d'autres enzymes; protéine constituant l'ADN polymérase; ADN contenant une séquence de bases codant pour l'ADN polymérase; et procédé de production de l'ADN polymérase. Celle-ci est une nouvelle polymérase ayant à la fois une forte capacité d'extension de l'amorce et une activité exonucléase 3'?5'.

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