Novel dna polymerase iii holoenzyme delta subunit nucleic...

Details Novel dna polymerase iii holoenzyme delta subunit nucleic... Novel dna polymerase iii holoenzyme delta subunit nucleic...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 9/12 (2006.01) C12Q 1/48 (2006.01)

Patent

CA 2416179

Gene and amino acid sequences encoding DNA polymerase III holoenzyme .delta. subunits and structural genes from bacteria are provided. Also provided are antibodies and other reagents useful to identify DNA polymerase III .delta. subunit molecules. Also provided are methods to identify DNA polymerase III .delta. subunit molecules. The use of DNA polymerase III .delta. subunit molecules in assays to identify candidate antibiotics are also provided.

L'invention concerne des séquences de gènes et d'acide aminés codant pour des sous-unités d'ADN-polymérase III/holoenzyme .delta. et des gènes de structure issus de bactéries. L'invention concerne également des anticorps, et d'autres réactifs utiles dans l'identification des molécules de sous-unités d'ADN-polymérase /holoenzyme .delta.. L'invention concerne, en outre, des méthodes d'identification des molécules de sous-unités d'ADN-polymérase III/holoenzyme .delta.. L'invention concerne enfin l'utilisation desdites molécules de sous-unités d'ADN-polymérase III/holoenzyme .delta. dans des dosages permettant d'identifier des antibiotiques candidats.

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