Novel efficient production process for capsular...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/54 (2006.01) A61K 39/02 (2006.01) A61K 39/09 (2006.01) C07K 14/31 (2006.01) C07K 14/315 (2006.01) C07K 14/32 (2006.01) C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12N 15/31 (2006.01) C12N 15/52 (2006.01) C12N 15/74 (2006.01)

Patent

CA 2591421

The current invention provides methods and means for heterologous expression, production and/or secretion of complex capsular polysaccharides in non- pathogenic, non-invasive Gram-positive bacteria. The invention in particular provides non- pathogenic, non- invasive Gram-positive bacteria capable of expression and/or secretion of heterologous, complex polysaccharides from a pathogenic bacterial species. Such bacteria and polysaccharides produced therein may be applied according to the invention to provide compositions for vaccination for the treatment and prevention of infectious bacterial diseases.

L'invention concerne des méthodes et des moyens d'expression hétérologues permettant de produire et/ou sécréter des polysaccharides capsulaires complexes dans des bactéries non pathogènes, non invasives, Gram-positives. L'invention concerne, en particulier, une bactérie non pathogène, non invasive, Gram-positive capable d'exprimer et/ou de sécréter des polysaccharides hétérologues complexes à partir d'espèces bactériennes pathogènes. Ces bactéries et les polysaccharides produits peuvent être appliqués selon l'invention afin de fournir des compositions de bactéries non pathogènes, non invasives Gram-positives vaccination permettant de traiter et de prévenir des maladies bactériennes infectieuses.

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