Novel elongase gene and method for producing...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) A01H 5/00 (2006.01) A61K 38/45 (2006.01) C07K 16/40 (2006.01) C11B 1/00 (2006.01) C11C 1/00 (2006.01) C12N 9/10 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01) C12Q 1/48 (2006.01) G01N 33/573 (2006.01)

Patent

CA 2399349

The invention relates to a novel elongase gene comprising the sequences cited in sequence SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5 and SEQ ID NO:7 or to homologues, derivatives or analogues thereof. The invention also relates to a gene construct containing these genes or homologues, derivatives or analogues thereof, and to its use. The invention additionally relates to the vectors or transgenic organisms containing an elongase gene comprising the sequence SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5 and SEQ ID NO:7 or containing homologues, derivatives or analogues thereof. The invention relates to the use of the elongase sequences alone or in combination with additional elongases and/or with additional fatty acid biosynthesis genes. The invention relates to a novel elongase gene comprising the sequence SEQ ID NO:1 or to homologues, derivatives or analogues thereof. The invention also relates to a method for producing multiple-unsaturated fatty acids and to a method for introducing DNA into organisms which produce large quantities of oils and, in particular, oils having a high content of unsaturated fatty acids. The invention further relates to an oil and/or to a fatty acid preparation having a high content of multiple-unsaturated fatty acids that contain at least two double bonds and/or to a triacylglycerin preparation having a high content of multiple-unsaturated fatty acids that contain at least two double bonds.

L'invention concerne un nouveau gène d'élongase comportant les séquences mentionnées dans la séquence SEQ ID n·1 ; SEQ ID n·3 ; SEQ ID n·5 ; SEQ ID n·7 ou ses homologues, dérivés ou analogues. L'invention concerne également un gène chimère contenant de gène ou ses homologues, dérivés ou analogues, ainsi que son utilisation. L'invention concerne également les vecteurs ou les organismes transgéniques qui contiennent un gène d'élongase comportant la séquence SEQ ID n·1 ; SEQ ID n·3 ; SEQ ID n·5 concerne par ailleurs l'utilisation des séquences de gène d'élongase, seules ou en combinaison avec d'autres élongases et/ou d'autres gènes de biosynthèse d'acide gras. L'invention concerne également un nouveau gène d'élongase comportant la séquence SEQ ID n·1 ou ses homologues, dérivés ou analogues. L'invention concerne de plus un procédé permettant de préparer des acides gras à insaturation multiple, ainsi qu'un procédé permettant d'introduire de l'ADN dans des organismes produisant de grandes quantités d'huiles et en particulier d'huiles à haute teneur en acides gras insaturés. L'invention concerne pour finir une préparation à base d'huile et/ou d'acides gras à haute teneur en acides gras à insaturation multiple comportant au moins deux liaisons doubles et/ou une préparation à base de triacylglycérine à haute teneur en acides gras comportant au moins deux liaisons doubles.

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