Novel endo-beta-1,4-glucanases

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/42 (2006.01) C12N 1/20 (2006.01)

Patent

CA 2374433

An enzyme exhibiting endo-~-1,4-glucanase activity which belongs to family 9 of glycosyl hydrolases is obtainable from or endogeneous to a strain belonging to the genus <i>Bacillus</i> such as <i>Bacillus licheniformis</i>, ATCC 14580; an isolated polynucleotide (DNA) molecule encoding an enzyme or enzyme core (the catalytically active domain of the enzyme) exhibiting endo-~-1,4- glucanase activity selected from (a) polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:1 from nucleotide 76 to nucleotide 1455 or from nucleotide 76 to nucleotide 1941, (b) polynucleotide molecules that encode a polypeptide being at least 75% identical to the amino acid sequence of SEQ ID NO:2 from amino acid residue 26 to amino acid 485 or from amino acid residue 26 to amino acid residue 646, and (c) degenerate nucleotide sequences of (a) or (b), the expressed endoglucanase enzyme being useful in various industrial applications.

L'invention concerne une enzyme présentant l'activité des endo-ß-1,4-glucanases, qui appartient à la famille 9 des glycosyl-hydrolases et que l'on peut obtenir à partir d'une souche ou endogène à une souche appartenant au genre <i>Bacillus</i>, telle que <i>Bacillus licheniformis</i>, ATCC 14580 ; une molécule de polynucléotide (ADN) isolé qui code une enzyme ou un noyau d'enzyme (le domaine à activité catalytique de l'enzyme) et qui présente une activité endo-ß-1,4-glucanase, laquelle molécule est choisie parmi (a) des molécules de polynucléotides comprenant une séquence nucléotidique telle qu'illustrée dans SEQ ID NO:1 du nucléotide 76 au nucléotide 1455 ou du nucléotide 76 au nucléotide 1941, (b) des molécules de polynucléotides codant un polypeptide identique au moins à 75 % à la séquence d'acides aminés de SEQ ID NO:2 du reste d'acide aminé 26 au reste d'acide aminé 485 ou du reste d'acide aminé 26 au reste d'acide aminé 646, et (c) des séquences nucléotidiques dégénérées de (a) ou (b), l'endoglucanase exprimée étant utile dans diverses applications industrielles.

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