Novel expression cassette for expressing genes in plant seed

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2348888

The invention relates to an expression cassette for expressing genes in plant seed and to the plasmids containing said expression cassette. The invention includes the production of transgenic plant cells containing said expression cassette and the use of the plasmids in said expression cassette for producing transgenic plants. The invention can be applied in the field of biotechnology, pharmaceutics and plant production. The aim of the invention is to provide a means for the seed-specific expression in transgenic plants in such a manner that it is suitable for the production of the desired substances. Another aim of the invention is to construct an expression cassette which allows stable expression of genes of substances to be produced in plant seed at a high expression rate. The inventive expression cassette comprises the following essential components: the promoter of the gene of the seed protein which is analogous to the sucrose binding protein (SBP), optionally the DNA sequence of a signal peptide, preferably that of the SBP signal peptide, a gene to be expressed, 3' termination sequences.

L'invention concerne une cassette d'expression permettant d'exprimer des gènes quelconques de graines de plantes et les plasmides contenant cette cassette d'expression. L'invention concerne également la production de cellules végétales transgéniques contenant cette cassette d'expression ainsi que l'utilisation des plasmides dans cette cassette d'expression pour la production de plantes transgéniques. L'invention s'applique en biotechnologie, en pharmacie et dans la production de plantes. L'invention vise à permettre l'expression spécifique aux graines dans les plantes transgéniques d'une manière convenant à la production de matière. Elle a pour objet de créer une cassette d'expression qui permette d'exprimer, de façon stable et avec un taux d'expression élevé, des gènes de la matière à produire dans les graines de plantes. La cassette d'expression selon l'invention contient les principaux constituants suivants: le promoteur du gène de la protéine de graine qui est similaire à la protéine de liaison du saccharose (SBP); éventuellement la séquence ADN d'une séquence-signal, de préférence, de la séquence-signal SBP; un gène à exprimer et des séquences de terminaison 3'.

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