Novel expression-regulating sequences and expression...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/56 (2006.01) C12N 1/15 (2006.01) C12N 9/02 (2006.01) C12N 9/24 (2006.01) C12N 9/42 (2006.01) C12N 15/53 (2006.01) C12N 15/80 (2006.01) C12P 19/14 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2405954

The invention pertains to novel proteins corresponding to Chrysosporium glycosyl hydrolases of families 7 and 10, exhibiting a minimum aminoacid identity of 70 and 75 %, respectively, with the amino acid sequence of SEQ ID No's 2 and 4, and to a protein corresponding to a Chrysosporium glyceraldehyde phosphate dehydrogenase, exhibiting at least 86 % amino acid identity with the partial amino acid sequence of SEQ ID No. 6. The invention further relates to nucleic acid sequences encoding these proteins, and especially to promoter sequences regulating the expression of the corresponding genes. The preferred host for expressing these genes is a fungus, especially a Chrysosporium strain.

La présente invention concerne de nouvelles protéines correspondant aux familles 7 et 10 des glycosyl-hydrolases de Chrysosporium, présentant respectivement une identité d'aminoacides minimum de 70 et de 75 %, la séquence d'aminoacides de la SEQ ID Numéros 2 et 4, et une protéine correspondant à une glycéraldéhyde phosphate déshydrogénase de Chrysosporium présentant au moins une identité d'aminoacides de 86 % avec la séquence partielle d'aminoacides de la SEQ ID No. 6. Cette invention concerne également des séquences nucléotidiques codant ces protéines et plus particulièrement des séquences promoteur qui régulent l'expression des gènes correspondants. L'hôte préféré pour exprimer ces gènes est un champignon, plus spécifiquement une souche de Chrysosporium.

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