Novel expression vectors

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/67 (2006.01) C12N 15/79 (2006.01)

Patent

CA 2440148

The present invention relates generally to expression vectors and their use in gene expression or gene regulation assays. More particularly, the present invention provides expression vectors and/or reporter vectors providing kinetics of protein expression with improved temporal correlation to promoter activity. Even more particularly, the invention provides expression vectors comprising a transcribable polynucleotide which comprises a sequence of nucleotides encoding an RNA element which modulates the stability of a transcript corresponding to said transcribable polynucleotide. The present invention provides, inter alia, novel vectors, useful for identifying and analysing cis- and trans-acting regulatory sequences/factors as well as vectors and genetically modified cell lines or organisms that are particularly useful for drug screening and drug discovery.

L'invention concerne en général de nouveaux vecteurs d'expression et leur utilisation dans des analyses d'expression génique et de régulation génique. Elle concerne en particulier des vecteurs d'expression et/ou des vecteurs rapporteurs permettant d'obtenir une cinétique d'expression de protéines présentant une meilleure corrélation temporelle avec l'activité du promoteur. L'invention concerne plus particulièrement des vecteurs d'expression comprenant un polynucléotide transcriptible qui présente une séquence de nucléotides codant un élément d'ARN qui module la stabilité d'un transcrit correspondant audit polynucléotide transcriptible. L'invention concerne, entre autres, d'autres vecteurs servant à identifier et à analyser les séquences/facteurs de régulation agissant en cis et trans, ainsi que des vecteurs et des lignées cellulaires ou des organismes génétiquement modifié(e)s qui sont particulièrement utiles pour le criblage et la découverte de médicaments.

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