Novel expression vectors and methods of use

Details Novel expression vectors and methods of use Novel expression vectors and methods of use

C - Chemistry, Metallurgy

12

N

C12N 15/67 (2006.01) C12N 15/65 (2006.01) C12N 15/86 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2234931

The present invention is related to vectors and methods for increasing the expression of a desired gene product. Preferably this invention is used with genes expressing proteins that are not well tolerated by mammalian cells or where high levels of expression are necessary. In certain preferred embodiments it can be used as part of a multi- tiered expression system and with methods of intracellularly targeting a molecule.

La présente invention se rapporte à des vecteurs et à des procédés qui permettent d'augmenter l'expression d'un produit génique désiré. De préférence, lesdits procédés s'appliquent à des gènes exprimant des protéines qui ne sont pas bien tolérées par des cellules mammaliennes ou dans lesquelles des niveaux d'expression élevés sont nécessaires. Dans certaines réalisations préférées, l'invention peut constituer une partie d'un système d'expression étagé et être associée à des procédés permettant de cibler intracellulairement une molécule.

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