Novel glycoglycerophospholipid, antibody thereagainst, and...

C - Chemistry – Metallurgy – 07 – K

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C07K 16/12 (2006.01) C07H 13/04 (2006.01) C07H 15/06 (2006.01) C07H 15/10 (2006.01) C12P 19/44 (2006.01) G01N 33/569 (2006.01) G01N 33/92 (2006.01)

Patent

CA 2191884

Mouse is immunized with an antigen of a lipid fraction originating from Mycoplasma fermentans. Its spleen cells are fused with mouse myeloma cells to prepare hybridomas. A hybridoma is selected, which produces a monoclonal antibody having reaction specificity to GGPL-III that is a phosphocholine--containing glycoglycerolipid specific to Mycoplasma fermentans. Mycoplasma fermentans is detected by using the obtained antibody.

Un hybridome produisant un anticorps monoclonal ayant une spécificité de réaction pour le GGPL-III, qui est un glycéroglycolipide contenant de la phosphocholine et spécifique du Mycoplasma fermentans, est sélectionné parmi les hybridomes préparés par l'immunisation d'une souris avec une fraction lipide de M. fermentans en tant qu'antigène et par la fusion de cellules de myélome de souris avec des splénocytes. On détecte le M. fermentans en utilisant l'anticorps produit.

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