Novel heteropolymeric protein production methods

Details Novel heteropolymeric protein production methods Novel heteropolymeric protein production methods

C - Chemistry, Metallurgy

12

P

195/1.29

C12P 21/00 (2006.01) C07K 14/575 (2006.01) C07K 14/59 (2006.01) C12N 15/00 (2006.01) C12N 15/67 (2006.01) C12N 15/79 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2018676

Cultured mammalian cells transfected with new vectors comprising full- length or partial .alpha. and .beta. subunit genomic DNA sequences produce significantly higher levels of dimeric glycoprotein hormone than do cells transfected with .alpha. and .beta. subunit cDNA sequences. In cases where only the cDNA clones are available, the cDNA sequences can be used in new expression vectors comprising introns or other important genomic regions from a homologous or heterologous source.

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