C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2487933
The present invention relates to methods for identifying analytes in a sample comprising the steps of: (a) incubating said analytes with a plurality of bipartite capture probes, said capture probes being immobilized in predefined regions on a solid substrate, and each capture probe consisting essentially of a first fragment which is at one end immobilized to said substrate and at the other end is complementary linked to a second fragment, wherein said second fragment comprises an extension fragment capable of identifying an analyte; (b) monitoring complex formation between sample analytes and extension fragments; (c) sequentially modifying complex formation conditions; allowing the release of captured analyte molecules from the substrate; and (d) detecting and identifying the released analytes. The present invention also relates to different uses of said methods as well as microarrays and kits for performing said methods.
Cette invention a trait à des techniques d'identification d'analysats dans un échantillon consistant, (a), à faire incuber les analysats avec plusieurs sondes de capture bipartites, immobilisées sur des zones prédéfinies d'un substrat solide, chacune d'elles étant principalement composée d'un premier fragment dont une extrémité est immobilisée sur le substrat et dont l'autre extrémité est liée, de manière complémentaire, à un second fragment, ce dernier étant pourvu d'un prolongement capable d'identifier un analysat, (b), à contrôler la formation d'un complexe entre les analysats et les prolongements des fragments, (c), à modifier successivement les conditions de formation du complexe, ce qui permet de libérer les molécules d'analysats du substrat et, (d), à détecter et à analyser les analysats libérés. Cette invention porte également sur les différentes mises en application de ces techniques ainsi que sur des micro-ensembles d'échantillons et des nécessaires à cet effet.
Borden Ladner Gervais Llp
Pamgene B.v.
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