Novel high throughput method of generating and purifying...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2509512

A method of generating substantially pure polynucleotides in a multiple- compartment container using a multiple-compartment purification filter is described. In particular, a method of generating cDNA or cRNA labeled with biotin for the ease of detection is described.

L'invention concerne un procédé permettant de produire des polynucléotides sensiblement purs dans un récipient à compartiments multiples qui utilise un filtre de purification à compartiments multiples. Elle concerne en particulier un procédé de production d'ADNc ou d'ARNc marqués à l'aide de biotine pour faciliter la détection.

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