Novel hydroxysteroid dehydrogenase gene for alteration of...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 1/00 (2006.01) A01H 3/00 (2006.01) A01H 5/00 (2006.01) C12N 9/04 (2006.01) C12N 15/53 (2006.01)

Patent

CA 2631781

The present invention describes a novel plant hydroxysteroid dehydrogenase gene, (HSD) that is involved in a variety of physiological processes in higher plants. The novel gene product has a degree of similarity to mammalian ~-11- hydroxysteroid dehydrogenase genes, known to control the conversion of steroids from active to inactive forms. In plants, the over expression of the HSD gene results in transgenic plants with significantly greater biomass and vigour, increased seed yield, increased tolerance to salt and a suppression of ABA mediated seed dormancy.

La présente invention concerne un nouveau gène d~hydroxystéroïde déshydrogénase (HSD) qui est mis à contribution dans une variété de processus physiologiques dans des plantes supérieures. Le nouveau produit génique possède un certain degré de similarité avec les gènes ß-11-hydroxystéroïde déshydrogénase des mammifères, connus pour réguler la conversion de stéroïdes de formes actives en des formes inactives. Dans les plantes, la surexpression du gène HSD produit des plantes transgéniques avec une biomasse et une vigueur nettement supérieures, un rendement supérieur en graines, une tolérance accrue au sel et une suppression de la dormance des graines induite par l~ABA.

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