C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/55 (2006.01) C12N 9/22 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2225611
The invention relates to a novel IS (insertion sequence) element characteristic of a virulent isolate (ET12/cb1A isolate) of Burkholderia cepacia. The IS element is a hybrid of two other IS elements commonly found in isolates of B. cepacia, namely IS402 [SEQ ID NO:2] and IS1356 [SEQ ID NO:3]. The IS hybrid IS element has the sequence shown in the Figure [SEQ ID NO:1]. The fact that the hybrid IS element is characteristic of the virulent isolate means that it can be used as an indicator of that isolate. Thus, the invention includes a method of testing for an ET12/cb1A isolate of B. cepacia by testing a sample for the presence of the hybrid IS element, e.g. by amplification of the IS element by PCR and identification of the amplified sequence.
L'invention se rapporte à un nouvel élément IS (séquence d'insertion) caractéristique d'un isolat virulent (isolat ET12/cb1A) de Burkholderia cepacia. L'élément IS est un hybride de deux autres éléments IS présents habituellement dans des isolats de Burkholderia cepacia, à savoir IS402 [SEQ ID NO:2] et IS1356 [SEQ ID NO:3]. L'élément IS hybride porte la séquence présentée dans la figure [SEQ ID NO:1]. Le fait que l'élément IS hybride caractérise l'isolat virulent signifie qu'il peut servir d'indicateur de cet isolat. Par conséquent, l'invention concerne également un procédé permettant de rechercher un isolat ET12/cb1A de B. cepacia par contrôle de la présence de l'élément IS hybride dans un échantillon, par exemple par amplification de l'élément IS par la PCR et l'identification de la séquence amplifiée.
Johnson Wendy M.
Rozee Kenneth R.
Tyler Shaun D.
Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Health
Kirby Eades Gale Baker
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