C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2497288
The present invention relates to methods for identifying analyte nucleic acids present in a sample comprising nucleic acid molecules, said method comprising the steps of: (a) providing a porous substrate, said substrate comprising a plurality of micro-channels, wherein said micro-channels have immobilized thereon a target molecule capable of binding to an analyte present in said sample; wherein said channels are further provided with analyte amplification components, a reporter system; and said sample; (b) amplifying analyte nucleic acid molecules present within said sample; and (c) allowing binding to take place between an amplified analyte nucleic acid obtained in step (b), said target molecule immobilized onto the channels of said substrate and said reporter system, wherein said reporter system allows detecting whether binding has occurred between said target molecule and said analyte nucleic acid. The present invention further relates to the use of said methods as well as kits for carrying out said methods.
L'invention porte sur des procédés d'identification d'acides nucléiques d'analyte présents dans un échantillon, comportant les étapes suivantes: (a) acquisition d'un substrat poreux présentant plusieurs microcanaux sur lesquels sont immobilisées des molécules cibles pouvant se fixer à un analyte présent dans ledit échantillon, lesdits canaux étant en outre pourvus de composants d'amplification de l'analyte, d'un système reporter et dudit échantillon. (b) amplification des molécules d'acide nucléique de l'analyte présent dans ledit échantillon; et (c) permettre les liaisons entre: l'acide nucléique amplifié en (b), ladite molécule cible immobilisée sur les canaux dudit substrat, et ledit système reporter lequel permet de détecter si une liaison s'est produite entre ladite molécule cible et ledit acide nucléique d'analyte. L'invention porte également sur l'utilisation desdits procédés, et sur des trousses permettant de les mettre en oeuvre.
Kievits Tim
Van Beuningen Marinus Gerardus Johannus
Borden Ladner Gervais Llp
Pamgene B.v.
LandOfFree
Novel integrated microarray analysis does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Novel integrated microarray analysis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Novel integrated microarray analysis will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1932758