Novel laccase enzyme and the gene encoding the enzyme

Details Novel laccase enzyme and the gene encoding the enzyme Novel laccase enzyme and the gene encoding the enzyme

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C07K 14/39 (2006.01) C11D 3/386 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12N 15/63 (2006.01) C12N 15/80 (2006.01) C12P 1/00 (2006.01) C12P 17/18 (2006.01) C12P 21/02 (2006.01) D21C 5/00 (2006.01) D21H 17/00 (2006.01)

Patent

CA 2409007

The invention relates to a new laccase enzyme, which can be isolated from the strains of the Melanocarpus genus, the M. albomyces strain in particular. The pH optimum of the enzyme is within 5-8 and the enzyme works at a temperature of 30-80 ~C. The isoelectric point of the enzyme is about 4.0 as determined by isoelectric focusing and the molecular weight about 80 kDa, defined by SDS- PAGE. The enzyme is especially well suited to applications, wherein the pH and temperature conditions are high. The invention also relates to a gene that encodes laccase enzyme, and a laccase enzyme produced by recombinant technology.

L'invention concerne une laccase, enzyme qui peut être isolée à partir de souches du genre Melanocarpus, en particulier de la souche M. albomyces. Le pH optimum de l'enzyme est compris entre 5 et 8 et l'enzyme agit à une température comprise entre 30 et 80 ·C. Son point isoélectrique est de 4,0 environ, déterminé par focalisation isoélectrique, et son poids moléculaire est de 80 kDa environ, défini par une électrophorèse SDS-PAGE. Cette enzyme est particulièrement bien adaptée à des applications dans lesquelles le pH et la température sont élevés. L'invention concerne aussi un gène qui code pour cette enzyme ainsi qu'une laccase produite à l'aide de technique de recombinaison.

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