Novel lipase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/55 (2006.01) C12N 1/20 (2006.01) C12N 9/20 (2006.01) C12P 7/62 (2006.01)

Patent

CA 2633847

The present invention provides a novel lipase with a molecular weight of about 32 kDa, which is produced by a strain belonging to the genus Tetrasphaera, as well as a gene encoding the same. This lipase has the ability to recognize a medium-chain fatty acid as a substrate. The present invention also provides a novel lipase with a molecular weight of about 40 kDa, which is produced by a strain belonging to the genus Tetrasphaera and has the ability to recognize both a medium--chain fatty acid and a long-chain fatty acid as substrates, as well as a polynucleotide encoding the same. The present invention further provides Tetrasphaera sp. strain NITE P-154. The lipase of the present invention can be used as an immobilized enzyme and is useful in fields such as production of digestants and/or flavorings, production of clinical laboratory reagents, detergent enzymes and/or fats, as well as production of optically active intermediates for agricultural chemicals and pharmaceutical preparations.

La présente invention concerne : une nouvelle lipase produite par une bactérie appartenant au genre Tetrasphaera et de masse molaire d'environ 32 kDa ; ainsi qu'un gène codant pour ladite lipase. La lipase est capable de reconnaître un substrat acide gras à chaîne moyenne. La présente invention concerne spécifiquement : une nouvelle lipase produite par une bactérie appartenant au genre Tetrasphaera, capable de reconnaître un substrat acide gras à chaîne moyenne ou à longue chaîne, et de masse molaire d'environ 40 kDa ; et un polynucléotide codant pour ladite lipase. La présente invention concerne également une souche NITE P-154 d'une bactérie appartenant au genre Tetrasphaera. La lipase peut être employée au titre d'enzyme immobilisée, dans des domaines tels que la production d'un agent facilitant la digestion ou d'un agent aromatique, la production d'un réactif pour tests cliniques, d'une enzyme pour détergent ou d'un dégraissant, et la production d'un intermédiaire optiquement actif pour la synthèse d'une substance agrochimique/pharmaceutique.

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