Novel lipases and uses thereof

Details Novel lipases and uses thereof Novel lipases and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 9/20 (2006.01) C07K 16/14 (2006.01) C12N 15/52 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2495198

The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel lipolytic enzyme from Aspergillus niger. The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel lipolytic enzyme as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a lipolytic enzyme according to the invention is genetically modified to enhance its activity and/or level of expression.

La présente invention concerne une séquence de polynucléotides nouvellement identifiée qui comprend un gène codant une nouvelle enzyme lipolytique issue de Aspergillus niger. L'invention se rapporte à une séquence nucléotidique de pleine longueur du nouveau gène, à la séquence d'ADNc comprenant la séquence de codage de pleine longueur de la nouvelle enzyme lipolytique, ainsi qu'à la séquence d'acides aminés de la protéine fonctionnelle de pleine longueur et aux équivalents fonctionnels de cette dernière. L'invention concerne aussi des procédés d'utilisation des enzymes précitées dans des processus industriels, et des procédés de diagnostic d'infections fongiques. L'invention porte enfin sur des cellules transformées au moyen d'un polynucléotide de l'invention et sur des cellules dans lesquelles on a génétiquement modifié une enzyme lipolytique de l'invention afin d'augmenter son activité et/ou son niveau d'expression.

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