Novel macrocyclic compounds as metalloprotease inhibitors

C - Chemistry – Metallurgy – 07 – D

Patent

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Details

C07D 273/02 (2006.01) A61K 31/395 (2006.01) A61K 31/41 (2006.01) A61K 31/44 (2006.01) A61K 31/495 (2006.01) A61K 31/535 (2006.01) C07D 413/12 (2006.01) C07D 413/14 (2006.01) C07D 417/12 (2006.01) C07D 521/00 (2006.01) C12Q 1/25 (2006.01) C12Q 1/37 (2006.01)

Patent

CA 2287923

This invention relates to macrocyclic molecules of formula (I) which inhibit metalloproteinases, including aggrecanase, and the production of tumor necrosis factor (TNF). The present invention also relates to pharmaceutical compositions comprising such compounds and to methods of using these compounds for the treatment of inflammatory diseases. A novel enzymatic assay was developed to detect potential inhibitors of aggrecanase. The assay uses active aggrecanase accumulated in media from stimulated bovine nasal cartilage (BNC) or related cartilage sources and purified cartilage aggrecan monomer or a fragment thereof as a substrate.

La présente invention concerne des molécules macrocycliques inhibant des métalloprotéases, y compris une aggrécanase, et la production d'un facteur de nécrose des tumeurs (TNF). En particulier, les composés sont des inhibiteurs de métalloprotéases intervenant dans la dégradation de tissus et des inhibiteurs de libération de facteur de nécrose des tumeurs. La présente invention concerne également des compositions pharmaceutiques comprenant de tels composés, ainsi que des méthodes d'utilisation de ces composés dans le traitement d'affections inflammatoires.

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