Novel mammalian calcium channels and related probes, cell...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) A61K 38/17 (2006.01) C07K 14/705 (2006.01) C07K 16/28 (2006.01) C12N 15/11 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2377931

Sequences and partial sequences for three types of mammalian (human and rat sequences identified) T-type calcium channel subunits which we have labeled as the .alpha.1G, .alpha.1H and .alpha.1I subunits are provided. Knowledge of the sequence of these calcium channels permits the localization and recovery of the complete sequence from human cells, and the development of cell lines which express the novel calcium channels of the invention. These cells may be used for identifying compounds capable of acting as agonists or antagonists to the calcium channels.

L'invention concerne des séquences et des séquences partielles destinées à trois types de sous-unités de canaux calciques de type T de mammifères (séquences identifiées humaines et murines). Nous avons désigné ces sous-unités sous le nom de .alpha.¿1G?, .alpha.¿1H? et .alpha.¿1I?. La connaissance de la séquence de ces canaux calciques permet, d'une part, de localiser et de récupérer la séquence complète contenue dans les cellules humaines et, d'autre part, de développer des lignées cellulaires exprimant ces nouveaux canaux calciques. Ces cellules peuvent être utilisées afin d'identifier des composés capables d'agir comme agonistes ou antagonistes sur ces canaux calciques.

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