Novel method for production of dna biochips and applications...

C - Chemistry – Metallurgy – 12 – Q

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12Q 1/68 (2006.01) C07B 61/00 (2006.01) C07H 21/00 (2006.01) C07K 1/107 (2006.01)

Patent

CA 2475696

The invention relates to a method for production of an activated biochip for covalent fixing of oligonucleotide probes to a solid support by means of a spacer compound of the NHS-PEG-VS type and biochips produced by the above method. The invention further relates to methods for detection of nucleic acids in a sample or methods for screening compounds which may be specifically bound to oligonucleotide probes in which said biochips are used. The invention also relates to detection kits for, quantitative or qualitative analysis of nucleic acids in a sample, comprising said biochips and use of the above as affinity matrix for purification of nucleic acids, for sequencing nucleic acids, for the qualitative or quantitative analysis of the expression of genes or for the study and detection of genetic polymorphism.

La présente invention est relative à un procédé de préparation d'une biopuce activée pour la fixation covalente de sondes oligonucléotidiques sur un support solide par l'intermédiaire d'un composé espaceur de type NHS-PEG-VS, ainsi que les biopuces susceptibles d'être obtenues par un tel procédé. L'invention comprend également des méthodes de détection d'acides nucléiques dans un échantillon ou des méthodes de criblage de composés capables de se fixer spécifiquement sur des sondes oligonucléotidiques dans lesquelles sont mises en oeuvre les biopuces selon l'invention. La présente invention a aussi pour objet des kits de détection, d'analyse quantitative ou qualitative d'acides nucléiques dans un échantillon, comprenant de telles biopuces ainsi que l'utilisation de ces dernières comme matrice d'affinité pour la purification d'acide nucléique, pour le séquençage d'acide nucléique, pour l'analyse qualitative ou quantitative de l'expression de gènes ou encore pour l'étude et la détection de polymorphisme génétique.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Novel method for production of dna biochips and applications... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Novel method for production of dna biochips and applications..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Novel method for production of dna biochips and applications... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1741788

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.