Novel method for screening and selecting enzymes

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/37 (2006.01) A23C 19/032 (2006.01) A23C 19/04 (2006.01) A23L 1/03 (2006.01) C12Q 1/44 (2006.01)

Patent

CA 2569922

The present invention relates to a method for identifying an enzyme of interest comprising screening a set of candidate enzymes on an application matrix, preferably on at least a medium throughput level, and selecting enzymes for their ability to produce a functionality change in the application matrix. In the application matrix screening method of the invention it is not necessary to define the enzyme activity to be screened prior to applying the screening method.

L'invention concerne un procédé qui permet d'identifier une enzyme d'intérêt, selon lequel on procède au criblage d'un ensemble d'enzymes candidates sur une matrice d'application, de préférence au moins à un niveau de débit moyen, et on choisit les enzymes pour leur capacité à produire un changement de fonctionnalité dans la matrice d'application. Selon le procédé de criblage sur matrice d'application de l'invention, il n'est pas nécessaire de définir l'activité enzymatique à cribler avant d'appliquer le procédé de criblage.

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