G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/68 (2006.01)
Patent
CA 2430174
The invention concerns a method for detecting a compound modulating complexing between RNA polymerase and a protein intervening during transcription, which consists in: incubating a mixture comprising RNA polymerase intervening during transcription and the compound to be detected; detecting, by a complexing test, the possible significant variation of the amount of complex formed between RNA polymerase and said protein relative to a control value corresponding to the amount of complex formed between RNA polymerase and said protein in the absence of any modulator; and deducing therefrom, when there is a significant variation as defined above, that there has been formation of a bond between said compound and RNA polymerase and/or the protein intervening during transcription, which results in a modulation of the complexing between RNA polymerase and the protein intervening during transcription.
L'invention concerne un procédé de détection d'un composé modulateur de la complexation entre l'ARN polymérase et une protéine intervenant au cours de la transcription, dans lequel: - on incube un mélange comprenant l'ARN polymérase intervenant au cours de la transcription et le composé soumis au procédé de détection, - on détecte, par un test de complexation, la variation significative éventuelle de la quantité de complexe formé entre l'ARN polymérase et la susdite protéine par rapport à une valeur de contrôle correspondant à la quantité de complexe formé entre l'ARN polymérase et la susdite protéine en l'absence de tout modulateur, et on en déduit, lorsqu'il y a une variation significative telle que définie ci-dessus, qu'il y a eu formation d'une liaison entre d'une part le susdit composé et d'autre part l'ARN polymérase et/ou la protéine intervenant au cours de la transcription, ce qui se traduit par une modulation de la complexation entre l'ARN polymérase et la protéine intervenant au cours de la transcription.
Leonetti Jean-Paul
Pau Bernard
Rouby Joelle
Centre National de La Recherche Scientifique
Goudreau Gage Dubuc
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