C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/073 (2010.01) C12N 5/0735 (2010.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/50 (2006.01)
Patent
CA 2558946
Provided are means and methods for producing embryoid bodies (EBs) from multi- or pluripotent cells. In particular, a method of generating embryoid bodies (EBs) is described comprising agitation of a liquid suspension culture of multi- or pluripotent cells in a container until generation of cell aggregates, optionally diluting the suspension, and further agitation of the suspension until formation of El3s. Furthermore, the present invention relates to the use of the novel culturing method and EBs obtained thereby for a variety of applications including genomics, diagnostic assays, teratogenic/embryotoxicological and pharmacological assays as well as for the provision of tissue grafts.
L'invention concerne des moyens et des procédés permettant de produire des corps embryoïdes (EBs) à partir de cellules multi- ou pluripotentes. L'invention concerne notamment un procédé permettant de produire des corps embryoïdes (EBs), selon lequel il est prévu d'agiter une culture en suspension liquide de cellules multi- ou pluripotentes dans un récipient, jusqu'à obtention d'agrégats cellulaires, éventuellement de diluer ladite suspension, puis de continuer à agiter ladite suspension jusqu'à formation de EBs. L'invention concerne en outre l'utilisation du nouveau procédé de culture et des EBs obtenus, pour une variété d'applications, y compris la génomique, les méthodes diagnostiques, les dosages tératogènes/embryotoxicologiques et pharmaceutiques, de même que la fourniture de greffes tissulaires.
Bohlen Heribert
Kettenhofen Ralf
Kolossov Eugen
Kopp Isabella
Schwengberg Silke
Axiogenesis Ag
Sim & Mcburney
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