C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/10 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01) C12N 15/86 (2006.01) C12Q 1/18 (2006.01)
Patent
CA 2237391
Methods are provided for obtaining primary cultures of human breast, colonic and prostate epithelial cells using a fibroblast co-culture tissue culture system. The methods can be used to obtain normal, precancerous and malignant epithelial cell cultures. Methods are also provided for deriving substantially pure epithelial cell strains from the primary cultures, and for immortalizing the cell strains to provide cell lines. The primary cultures, cell strains and cell lines are used in biological and biochemical in vitro screening and/or diagnostic assays.
L'invention concerne des procédés d'obtention de cultures primitives de cellules épithéliales du sein, colique et prostatique chez l'homme, à l'aide d'un système de culture tissulaire en co-culture avec du fibroblaste. On peut utiliser les procédés pour obtenir des cultures cellulaires épithéliales normales, précancéreuses et malignes. L'invention concerne également des procédés permettant de dériver des souches des cellules épithéliales sensiblement pures à partir des cultures primitives, et d'immortaliser les souches de cellules afin de produire des lignées cellulaires. Les cultures primitives, les souches de cellules et les lignées cellulaires sont utilisées dans des titrages de criblage et/ou de diagnostic in vitro biochimiques.
Anderson Christine B.
Jerominski Leslie
Matsunami Norisada
Prescott Stephen
White Raymond L.
Gowling Lafleur Henderson Llp
University Of Utah
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