C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/09 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/533 (2006.01)
Patent
CA 2478173
It is intended to provide a method of highly sensitively analyzing SNPs. More specifically, it is intended to provide an SNP analysis method whereby a large amount of SNPs can be quickly analyzed using a DNA sample in a trace amount. An SNP analysis method by the invader method with the use of a FRET probe having a luminescent dye and a quencher characterized by using, as the luminescent dye in the FRET probe, a rare earth fluorescent complex label made of a rare earth element such as europium or terbium. A composition for controlling fluorescent luminescence which comprises a combination of a specific rare earth fluorescent complex label with a specific fluorescent quencher. A highly sensitive labeling probe characterized by using a rare earth fluorescent complex label as a luminescent dye and a fluorescent quencher label as a quencher in a highly sensitive labeling probe such as a FRET probe and an SNP analysis kit containing the same.
L'invention concerne un procédé d'analyse de SNPs extrêmement précise. Elle concerne, plus particulièrement, un procédé permettant d'analyser rapidement une quantité importante de SNPs au moyen d'une trace de spécimen d'ADN. Ce procédé consiste à mettre en application une technique invasive par l'intermédiaire d'une sonde FRET contenant un colorant luminescent à base d'un complexe fluorescent constitué par un élément des terres rares, tel qu'europium ou terbium, et par un extincteur. Elle concerne également une composition servant à contrôler la fluoroluminescence et constituée par un complexe fluorescent spécifique des terres rares associé à un extincteur de fluorescence spécifique. Elle concerne, de plus, une sonde de marquage très sensible, telle qu'une sonde FRET, mettant en application un complexe fluorescent des terres rares sous forme de colorant luminescent, ainsi qu'un extincteur de luminescence. Elle concerne enfin une trousse d'analyse SNP contenant cette sonde.
Matsumoto Kazuko
Yuan Jingli
Goudreau Gage Dubuc
Matsumoto Kazuko
Mitsubishi Rayon Co. Ltd.
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