Novel method of regulating seed development in plants and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/29 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2343978

The present invention provides a method of inducing seed development in plants, preferably in the absence of sexual fertilisation, said method comprising inhibiting or preventing the expression of one or more regulatory polypeptides that otherwise prevent asexual seed development in plants. The invention further provides novel genetic sequences (that is FIS1, FIS2, FIS3). The invention further provides transformed plants having a wide range of novel phenotypes including, but not limited to, the ability to reproduce asexually, develop seed in the absence of fertilization, and the ability to produce parthenocarpic fruit or seedless fruit or fruits with soft seed traces such that the fruit are marketable as less seedy than wild-type fruit or seedless. The isolated nucleic acid molecules are further useful in the detection of proteins and genetic sequences which interact with the polypeptides encoded by said nucleic acid molecules in the regulation of seed development in plants.

L'invention concerne un procédé visant à déclencher le développement de graines chez des végétaux, de préférence sans fécondation sexuelle, ledit procédé comportant l'étape consistant à inhiber ou à prévenir l'expression d'un ou de plusieurs polypeptides régulatoires qui, sans cela, empêcheraient le développement de graines asexuées chez les végétaux. L'invention concerne en outre de nouvelles séquences génétiques (à savoir FIS1, FIS2, FIS3). L'invention concerne de plus des plantes transformées possédant une large gamme de nouveaux phénotypes comprenant notamment, mais pas exclusivement, la capacité de se reproduire de manière asexuée, de développer des graines sans fécondation, et la capacité de produire des fruits parthénocarpiques, ou des fruits sans pépins, ou des fruits contenant des traces de graines molles, qui permettent de commercialiser les fruits comme fruits contenant moins de pépins que des fruits de type sauvage ou sans pépins. Les molécules d'acides nucléiques isolées sont en outre utiles pour détecter des protéines et des séquences génétiques qui interagissent avec les polypeptides codés par lesdites molécules d'acides nucléiques dans la régulation du développement de graines chez des végétaux.

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