Novel n-acetylgalactosamine transferases and nucleic acids...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) C07K 16/40 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12P 21/08 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2495177

An enzyme which transfers N-acetylgalactosamine to N-acetylglucosamine via a .beta.1-4 linkage was isolated and the structure of its gene was explained. This led to the production of said enzyme or the like by genetic engineering techniques, the production of oligosaccharides using said enzyme, and the diagnosis of diseases on the basis of said gene or the like.The present invention uses a protein having the amino acid sequence shown in SEQ ID NO: 1, 3, 26 or 27 in the Sequence Listing or a variant of said amino acid sequence wherein one or more acids are substituted or deleted, or one or more acids are inserted or added and having the activity of transferring N- acetylgalactosamine (GalNAc) to N-acetylglucosamine serving as a substrate via a .beta.1-4 linkage and nucleic acids encoding said protein.

Selon l'invention, une enzyme transformant N-acétylgalactosamine en N-acétylglucosamine par l'intermédiaire d'une liaison .beta.1-4 a été isolée, et la structure du gène codant cette enzyme a été étudiée. Ainsi, il a été possible de produire cette enzyme ou analogues au moyen de procédés de modification génétique, de produire des oligosaccharides au moyen de cette enzyme, et de diagnostiquer des maladies sur la base dudit gène ou analogue. La présente invention fait intervenir une protéine présentant la séquence d'acides aminés indiquée dans SEQ ID NO : 1, 3, 26 ou 27 dans la liste de séquences, ou un variant de ladite séquence d'acides aminés dans lequel un ou plusieurs acides sont substitués ou supprimés, ou un ou plusieurs acides sont insérés ou additionnés. Ladite protéine présente l'activité consistant à transformer N-acétylgalactosamine (GalNAc) en N-acétylglucosamine servant de substrat, par l'intermédiaire d'une liaison .beta.1-4. L'invention concerne également des acides nucléiques codant ladite protéine.

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