C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/12 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/00 (2006.01) C12N 9/10 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2485142
An enzyme having an activity of transferring N-acetylglucosamine to the non- reducing end of a Gal.beta.1-4Glc or Gal.beta.1-4GlcNAc-group via a .beta.-1,3 bond; a nucleic acid encoding the same; and a method of diagnosing cancer and/or tumor, in particular, digestive cancer and/or tumor using the expression dose of a gene of the above enzyme as an indication. A gene of a novel enzyme having an activity of transferring N-acetylglucosamine to the non- reducing end of a Gal.beta.1-4Glc or Gal.beta.1-4GlcNAc-group via a .beta.-1,3 bond is cloned from human stomach cells and its base sequence is determined. Then this enzyme is expressed. Since this enzyme is scarcely or never produced in cancer and/or tumor, in particular, digestive cancer and/or tumor cells, cancer and/or tumor can be diagnosed with the use of the expression of the enzyme gene as an indication.
L'invention concerne une enzyme à activité de transfert de N-acétylglucosamine à l'extrémité non réductible d'un groupe Gal.beta.1-4Glc ou Gal.beta.1-4GlcNAc via une liaison .beta.-1,3. L'invention concerne également un acide nucléique codant cette enzyme et un procédé de diagnostic de cancer et/ou de tumeur, en particulier du tube digestif, grâce à l'expression d'un gène de ladite enzyme, qui sert d'indication. On réalise le clonage d'un gène de l'enzyme à activité de transfert de N-acétylglucosamine à l'extrémité non réductible d'un groupe Gal.beta.1-4Glc ou Gal.beta.1-4GlcNAc via une liaison .beta.-1,3, depuis des cellules stomacales humaines, puis on détermine la séquence de base du résultat de ce clonage. Vient ensuite l'expression de l'enzyme, laquelle se produit rarement ou ne se produit jamais en cas de cancer et/ou de tumeur, en particulier du tube digestif, moyennant quoi un diagnostic de cancer et/ou de tumeur est possible grâce à l'expression d'un gène de ladite enzyme, qui sert d'indication
Inaba Niro
Narimatsu Hisashi
Togayachi Akira
Fujirebio Inc.
National Institute Of Advanced Industrial Science And Technology
Robic
LandOfFree
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