Novel neomycin-phosphotransferase-genes and methods for the...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/85 (2006.01) C12N 5/10 (2006.01) C12N 9/12 (2006.01) C12N 15/54 (2006.01) C12N 15/79 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2507664

The invention relates to novel modified neomycin phosphotransferase genes and the use thereof in a selection method for high-producing recombinant cells. The invention also relates to expression vectors containing a modified neomycin phosphotransferase gene and a gene of interest in a functional bond with a heterologous promoter, and a method for producing heterologous gene products by using said expression vectors.

L'invention concerne des gènes modifiés de la néomycine phosphotransférase et leur utilisation dans un procédé de sélection de cellules recombinantes hautement productives. La présente invention porte également sur des vecteurs d'expression contenant un gène modifié de la néomycine phosphotransférase et un gène d'intérêt en liaison fonctionnelle avec un promoteur hétérologue, ainsi que sur un procédé pour produire des produits géniques hétérologues au moyen de ces vecteurs d'expression.

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