Novel nucleic acid probe and novel method of assaying...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2424614

A novel nucleic acid probe for simultaneously assaying nucleic acids with a simple apparatus in case where one or more nucleic acids are present in the assay system, characterized in that, in a single-stranded nucleic acid probe labeled with a plural number of fluorescent pigments so as to form at least one pair of fluorescent pigments causing the FRET phenomenon (i.e., a fluorescent pigment capable of serving as a donor pigment and another fluorescent pigment capable of serving as an acceptor pigment), the base sequence is designed so that the fluorescent intensity of the acceptor pigment is weakened when the probe is hybridized with the target nucleic acids, and the above pigments are labeled.

L'invention concerne une nouvelle sonde d'acide nucléique permettant d'effectuer des essais d'acides nucléiques simultanés au moyen d'un appareil simple, dans le cas où un ou plusieurs acides nucléiques sont présents dans un système d'essai. L'invention est caractérisée en ce que, dans une sonde d'acide nucléique monocaténaire marquée par plusieurs pigments fluorescents, de sorte à former au moins une paire de pigments fluorescents pouvant provoquer le phénomène FRET (à savoir un pigment fluorescent peut être utilisé en tant que pigment donneur, et un autre pigment fluorescent peut être utilisé en tant que pigment receveur), la séquence de base est désignée de sorte que l'intensité fluorescente du pigment receveur soit affaiblie lorsque la sonde est hybridée avec les acides nucléiques cibles, et que les pigments mentionnés ci-dessus sont marqués.

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