Novel omega-3 fatty acid desaturase family members and uses...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/04 (2006.01) C12N 5/10 (2006.01) C12N 9/02 (2006.01) C12N 15/53 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2597225

The present invention relates to the isolation of a second omega-3 fatty acid desaturase (FAD3) gene in flax (Linum usitatissimum) and its promoter region, as well as the identification of FAD3 mutant alleles, and the development of markers for wild type and mutated alleles. The FAD3 genes encode the enzymes which control omega-3 fatty acid desaturation and, thus, the levels of linolenic acid (18:3(n-3)). Accordingly, the molecules of the present invention can be utilized, for example, to develop markers for the mutations in flax FAD3 genes and for seed-specific modification of fatty acid and protein compositions in plant seeds.

L'invention concerne l'isolation d'un second gène de la désaturase d'acides gras oméga-3 (FAD3) dans une fibre de lin (Linum usitatissimum) et sa région promoteur, ainsi que l'identification d'allèles mutants de FAD3 et le développement de marqueurs d'allèles de type sauvage et ayant mutés. Les gènes FAD3 codent pour des enzymes qui commandent la désaturation des acides gras oméga-3 et donc, les niveaux d'acide linolénique (18:3(n-3)). En conséquence, on peut utiliser les molécules de l'invention, par exemple, pour développer des marqueurs destinés aux mutations dans les gènes FAD3 d'une fibre de lin et pour modifier spécifiquement des graines de compositions d'acides gras et de protéines dans les graines de plantes.

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