Novel oxidoreductases and uses thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 9/02 (2006.01) A21D 8/04 (2006.01) A23C 9/12 (2006.01) C07K 16/40 (2006.01) C12N 15/53 (2006.01) C12N 15/63 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2641455

The invention relates to newly identified polynucleotide sequences comprising a gene that encodes a novel oxidoreductase isolated from Aspergillus niger. The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel oxidoreductase as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in baking and in dairy applications. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a oxidoreductase according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.

L'invention concerne des séquences polynucléotidiques nouvellement identifiées comprenant un gène codant pour une nouvelle oxydoréductase isolée à partir d'Aspergillus niger. L'invention concerne la séquence nucléotidique pleine longueur de ce nouveau gène, la séquence d'ADN complémentaire comprenant la séquence de codage pleine longueur de la nouvelle oxydoréductase ainsi que la séquence d'acides aminés de la protéine fonctionnelle pleine longueur et des équivalents fonctionnels correspondants. L'invention se rapporte en outre à des méthodes d'utilisation de ces enzymes dans des applications de cuisson au four et de laiterie. L'invention concerne également des cellules transformées avec un polynucléotide de l'invention et des cellules dans lesquelles une oxydoréductase de l'invention est modifiée génétiquement en vue d'un renforcement ou d'une réduction de son activité et/ou de son niveau d'expression.

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