Novel peptides derived from autoantigen for use in...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/47 (2006.01) A61K 38/04 (2006.01) A61K 38/17 (2006.01) C07K 7/04 (2006.01) C07K 7/06 (2006.01) C07K 7/08 (2006.01) C12Q 1/02 (2006.01) G01N 33/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2173874

The present invention relates to novel peptides derived from the autoantigen HC gp-39, said peptides comprising at least one of the amino acid sequences FGRSFTLAS (SEQ ID No.1), FTLASSETG (SEQ ID No. 2), YDDQESVKS (SEQ ID No.3) and FSKIASNTQ (SEQ ID No.4). The peptides resemble MHC Class II restricted T-cell epitopes present on the autoantigen HC gp-39 in articular cartilage. HC gp-39 and said peptides can be used in antigen-specific treatment of articular cartilage destruction in autoimmune diseases to induce tolerance of the immune system. The autoantigen HC gp-39 and said peptides are also suitable to induce arthritis in non-human animals, preferably mice. The invention furthermore relates to pharmaceutical compositions comprising said autoantigen and/or said peptides, a diagnostic method for the detection of autoreactive T cells in a test sample and test kits to be used in said method.

Nouveaux peptides dérivés de l'autoantigène HC gp-39, lesdits peptides comportant au moins une des séquences d'acides aminés FGRSFTLAS (SEQ ID No. 1), FTLASSETG (SEQ ID No. 2), YDDQESVKS (SEQ ID No. 3) et FSKIASNTQ (SEQ ID No. 4). Les peptides ressemblent aux épitopes de lymphocytes T limités de la Classe II du CMH, présents sur l'autoantigène HC gp-39 dans le cartilage articulaire. Le HC gp-39 et lesdits peptides sont utilisables dans un traitement spécifique d'antigène de la destruction du cartilage articulaire dans les maladies auto-immunes, pour induire la tolérance du système immunitaire. L'autoantigène HC gp-39 et lesdits peptides peuvent également servir à induire l'arthrite dans les animaux autres que l'homme, et de préférence dans les souris. On a également prévu des compositions pharmaceutiques comportant ledit autoantigène et/ou lesdits peptides, un procédé diagnostique de détection de lymphocytes T autoréactifs dans un échantillon testé, et des trousses d'essai utilisables selon ce procédé.

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