C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/079 (2010.01)
Patent
CA 2751497
The present invention relates to a permanent human cell line comprising a nucleic acid sequence for the adenoviral gene functions E1A and E1B and the nucleic acid sequence for the SV40 large T-antigen or the Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA- 1). Further, the present invention relates to a method for transient expression of recombinant polypeptides and proteins in said permanent human cell line.
La présente invention concerne une lignée cellulaire humaine permanente comprenant les séquences d'acide nucléique pour les fonctions géniques adénovirales E1A et E1B et la séquence d'acide nucléique pour l'antigène grand T de SV40 ou l'antigène nucléaire 1 du virus Epstein-Barr (EBV) (EBNA-1). La présente invention concerne également un procédé d'expression transitoire de polypeptides et protéines recombinants dans la lignée cellulaire humaine permanente.
Schiedner Gudrun
Volpers Christoph
Borden Ladner Gervais Llp
Cevec Pharmaceuticals Gmbh
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