Novel phospholipases and uses thereof

Details Novel phospholipases and uses thereof Novel phospholipases and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 9/20 (2006.01) A21D 8/04 (2006.01) C07K 16/40 (2006.01) C12N 5/00 (2006.01) C12N 15/62 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2486529

The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel phospholipase isolated from Aspergillus niger. The invention features the full length nucleotide sequence of the novel gene, the cDNA sequence comprising the full length coding sequence of the novel phospholipase as well as the amino acid sequence of the full-length functional protein and functional equivalents thereof. The invention also relates to methods of using these enzymes in industrial processes and methods of diagnosing fungal infections. Also included in the invention are cells transformed with a polynucleotide according to the invention and cells wherein a phospholipase according to the invention is genetically modified to enhance or reduce its activity and/or level of expression.

L'invention concerne une séquence polynucléotidique nouvellement identifiée qui comprend un gène codant pour une nouvelle phospholipase isolée de Aspergillus niger. L'invention présente la séquence nucléotidique complète du nouveau gène, la séquence ADN complémentaire comprenant la séquence de codage complète de la nouvelle phospholipase ainsi que la séquence d'acide aminé de la protéine fonctionnelle complète et leurs équivalents fonctionnels. L'invention concerne également des méthodes d'utilisation de ces enzymes dans des processus industriels et des méthodes permettant de diagnostiquer des infections fongiques. L'invention concerne enfin des cellules transformées à l'aide d'un polynucléotide selon l'invention et des cellules dans lesquelles une phospholipase selon l'invention est génétiquement modifiée afin de stimuler ou de diminuer son activité et/ou son taux d'expression.

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