Novel plant acetylenase and use thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) A01H 5/00 (2006.01) C11B 1/00 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 9/02 (2006.01) C12N 15/80 (2006.01) C12N 15/82 (2006.01) C12P 7/64 (2006.01)

Patent

CA 2250234

The present invention relates to a novel plant enzyme called delta (12) fatty acid acetylenase. This enzyme is responsible for the conversion of fatty acids to acetylenic acids and the invention relates to production of such acids. The invention also relates to use of cDNA encoding acetylenase, preferably Crepis alpina delta (12) acetylenase, for transforming organisms such as oil accumulating organisms selected from the group consisting of oil crops, oleogeneous yeasts and moulds. Furthermore, the invention relates to organisms such as oil accumulating organisms transformed with acetylenase cDNA, and to oils and other acetylenic compounds from said organisms.

Nouvelle enzyme végétale appelée acétylénase d'acide gras delta 12, responsable de la transformation des acides gras en acides acétyléniques. L'invention concerne la production de tels acides, ainsi que l'utilisation d'ADNc codant l'acétylénase, de préférence l'acétylénase delta 12 de Crepis alpina, pour transformer des organismes tels que les organismes oléagineux choisis dans le groupe constitué par les plantes oléagineuses cultivées, les moisissures et les levures oléogènes. De plus, l'invention concerne des organismes tels que les organismes oléagineux transformé au moyen de l'ADNc codant l'acétylénase, ainsi qu'aux huiles et autres composés acétyléniques provenant de ces organismes.

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