Novel polycyclic aromatic fluorogenic substrates for hydrolases

Details Novel polycyclic aromatic fluorogenic substrates for hydrolases Novel polycyclic aromatic fluorogenic substrates for hydrolases

C - Chemistry, Metallurgy

12

Q

C12Q 1/42 (2006.01) C07C 309/43 (2006.01) C09B 3/82 (2006.01) C09B 57/00 (2006.01) C12Q 1/34 (2006.01)

Patent

CA 2335564

The subject invention provides compounds useful as fluorogenic substrates for the hydrolytic enzymes. Upon hydrolysis of the hydrolyzable group, a halo- pyrene substituted molecule is developed which is highly fluorescent, water soluble and exhibits several desirable characteristics, including a large Stokes' shift.

La présente invention concerne de nouveaux composés utilisés comme substrats fluorogènes pour les enzymes hydrolytiques. Lors de l'hydrolyse du groupe hydrolysable, on obtient une molécule substituée par halo-pyrène hautement fluorescente, hydrosoluble et présentant de nombreuses caractéristiques souhaitées, notamment un important déplacement de Stokes.

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