C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) A01N 65/00 (2009.01) A01P 1/00 (2006.01) C07H 21/00 (2006.01) C07K 1/18 (2006.01) C07K 1/30 (2006.01) C07K 14/375 (2006.01) C12N 9/02 (2006.01) C12N 15/31 (2006.01) C12N 15/63 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2351894
Attempts are made to find out and identify a novel antibacterial protein whereby the growth of plant pathogenic bacteria, for example, Pyricularia orizae and Rhizoctonia solani, which are causative of the two major diseases of rice, can be inhibited at a relatively low concentration, and to clone the gene of this protein. Thus, an antibacterial protein, which can be obtained from a fraction prepared by extracting Lyophyllum shimeji with water and subjecting the extract to ammonium sulfate precipitation, exerts an antibacterial activity at least on Pyricularia orizae or Rhizoctonia solani, and contains components of about 70 kDa and/or about 65 kDa in molecular weight when determined by the SDS-PAGE method, is provided.
On a essayé de trouver et d'identifier une nouvelle protéine antibactérienne, grâce à laquelle la croissance de bactéries pathogènes de plantes, par exemple, Pyricularia orizae et Rhizoctonia solani, induisant les deux maladies principales du riz, peut être inhibée à une concentration relativement faible, et de cloner le gène de cette protéine. L'invention concerne une protéine antibactérienne pouvant être obtenue à partir d'une fraction préparée par extraction de Lyophyllum shimeji, avec de l'eau, et par exposition de l'extrait à un précipité de sulfate d'ammonium, qui exerce une activité antibactérienne au moins surPyricularia orizae ou Rhizoctonia solani, et qui contient des composants présentant un poids moléculaire d'environ 70 kDa et /ou environ 65 kDa lorsqu'il est déterminé par la méthode SDS-PAGE.
Inoue Yasuhiro
Kuwata Shigeru
Takakura Yoshimitsu
Forestry And Fisheries Corporate Juridical Person Society For Techno-Innovation Of Agri
Japan Tobacco Inc.
Robic
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