Novel receptor protein and method for the diagnosis of an...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) C07K 14/705 (2006.01) C07K 16/28 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2341154

A novel seven-pass transmembrane receptor protein found out in immature dendritic cells; a DNA encoding this protein; a replicable recombinant DNA obtained by integrating the above DNA into a replicable expression vector; microbial or cultured cells transformed by the replicable recombinant DNA as described above; a seven-pass transmembrane receptor protein produced on the cell surface of the above transformant; a method for screening a ligand to the above seven-pass transmembrane receptor protein or a substance preventing the ligand from binding to the seven-pass transmembrane receptor protein; an antibody capable of binding to the seven-pass transmembrane receptor protein; and a method for diagnosing inflammatory diseases such as rheumatism which involves the step of measuring the expression dose of the seven-pass transmembrane receptor protein expressed in human leukocytes.

Nouvelle protéine de récepteur transmembranaire à sept canaux découverte dans des cellules dendritiques immatures; ADN codant cette protéine; ADN de recombinaison apte à la réplication obtenu par intégration de l'ADN ci-dessus dans un vecteur d'expression apte à la réplication; cellules microbiennes ou cultivées transformées par cet ADN de recombinaison apte à la réplication; protéine de récepteur transmembranaire à sept canaux produite sur la surface d'une cellule transformée; procédé servant à rechercher par criblage un ligand de ladite protéine de récepteur transmembranaire à sept canaux ou une substance empêchant la fixation du ligand à ladite protéine; anticorps capable de se fixer à cette protéine de récepteur transmembranaire à sept canaux; procédé servant à diagnostiquer des maladies inflammatoires, telles que des rhumatismes, et consistant à mesurer la dose d'expression de ladite protéine de récepteur transmembranaire à sept canaux dans des leucocytes humains.

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