Novel replication process

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/00 (2006.01) A61K 39/145 (2006.01) C07K 14/11 (2006.01) C12N 7/02 (2006.01)

Patent

CA 2205677

The proposed process permits replication of human influenza virus at a low multiplicity of infection in a Vero cell line by maintaining a trypsin concentration of at least 0.05 µg/ml of the culture medium throughout the growth cycle.

Procédé permettant de répliquer un virus de la grippe humaine à une faible multiplicité d'infection dans une lignée cellulaire véro en maintenant une concentration de trypsine d'au moins 0,05µg/ml du milieu de culture pendant toute la durée du cycle de croissance.

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