Novel replication process

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 7/00 (2006.01)

Patent

CA 2205410

The proposed process permits replication of human influenza virus at a low multiplicity of infection in a Vero cell line by maintaining a trypsin concentration of at least 0.05 µg/ml of the culture medium throughout the growth cycle.

Le procédé décrit est destiné à la réplication du virus de la grippe humaine selon une faible multiplicité d'infection dans une lignée cellulaire Véro. Ce procédé consiste à maintenir une concentration de trypsine d'au moins 0,05 µg/ml dans le milieu de culture pendant tout le cycle de croissance du virus.

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