Novel rubella e1 envelope protein variants and their use in...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/19 (2006.01) C12N 15/14 (2006.01) C12N 15/62 (2006.01) G01N 33/53 (2006.01) G01N 33/569 (2006.01) C07K 19/00 (2006.01)

Patent

CA 2705716

The invention relates to soluble Rubella E1 antigens and variants of these antigens. The antigens comprise amino acids 201 to 432 or 169 to 432 and are lacking amino acids 453 to 481 as well as at least the amino acids 143 to 164. They further contain a region spanning two disulfide-bridges. The invention also relates to a recombinant DNA molecule encoding said Rubella E1 antigens, the expression of Rubella E1 antigens as chaperone fusion proteins and their use in a method of detecting antibodies against Rubella in a sample.

L'invention porte sur des antigènes E1 de rubéole solubles et sur des variants de ces antigènes. Les antigènes comprennent des acides aminés 201 à 432 ou 169 à 432 et n'ont pas les acides aminés 453 à 481 ainsi qu'au moins les acides aminés 143 à 164. Ils contiennent en outre une région s'étendant entre deux ponts disulfure. L'invention porte également sur une molécule d'ADN recombinant codant pour lesdits antigènes E1 de rubéole, l'expression d'antigènes E1 de rubéole en tant que protéines de fusion chaperonnes et sur leur utilisation dans un procédé de détection d'anticorps dirigés contre la rubéole dans un échantillon.

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