Novel selectable marker genes

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) A01H 1/00 (2006.01) A01H 5/00 (2006.01) A01N 57/20 (2006.01) A01P 13/00 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2671365

The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.

L'invention concerne un nouveau gène désigné ici par DSM-2. Ce gène a été identifié dans Streptomyces coelicolor A3. La protéine DSM-2 a une parenté lointaine avec PAT et BAR. L'invention concerne également des gènes optimisés pour les plantes codant des protéines DSM-2 ; DSM-2 peut être utilisé comme un caractère transgénique pour conférer aux plantes et aux cellules végétales une tolérance aux herbicides glufosinate et bialaphos. Une utilisation préférée des gènes sujets est celle de marqueurs sélectionable. L'utilisation de ce gène comme marqueur sélectionnable dans un système bactérien peut augmenter leur efficacité de transformation des plantes. L'utilisation de DSM-2 comme unique marqueur de sélection élimine la nécessité d'un autre marqueur antibiotique médical (tel que la résistance à l'ampicilline) pendant le clonage. Diverses autres utilisations sont également possibles selon l'invention.

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