Novel shuttle vector

Details Novel shuttle vector Novel shuttle vector

C - Chemistry, Metallurgy

12

N

C12N 15/74 (2006.01) A61K 35/74 (2006.01) A61K 38/46 (2006.01) A61K 48/00 (2006.01) A61P 35/00 (2006.01) C12N 1/21 (2006.01) C12N 15/09 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2586126

A bifidobacteria microorganism (BM)/E. coli shuttle vector, which has a wide host range in BM, yields a large copy number and allows the overexpression of a desired protein when it is used as an expression vector; an expression vector that allows the expression of a desired gene in BM using the shuttle vector; BM transformed with the expression vector; and an antitumor agent containing the BM as an active ingredient are provided. The BM/E. coli shuttle vector which contains the replication origin (oriV)-repB region of pTB6, a region derived from pTB6 not containing MembB, MobA, OrfI and oriT regions, the replication origin (oriC) region of E. coli, and a plasmid region derived from E. coli in which DNA encoding the N-terminal region of .beta.-lactamase which is an expression product of an ampicillin resistance gene (ampR) has been deleted, and whose average copy number is 6 to 30 is constructed.

L~invention a trait à un vecteur navette micro-organisme bifidobactérien (BM)/E. coli qui couvre une large gamme d~hôtes parmi les BM, produit un grand nombre de copies et permet la surexpression d~une protéine voulue lorsqu~il est utilisé en tant que vecteur d~expression ; à un vecteur d~expression permettant l~expression d~un gène voulu dans un BM au moyen dudit vecteur navette ; à un BM transformé par le vecteur d~expression et à un agent antitumoral contenant le BM qui en constitue un ingrédient actif. Est construit le vecteur navette BM/E. coli contenant la région d~origine de la réplication (oriV)-repB de pTB6, une région dérivée de pTB6 ne contenant pas les régions MembB, MobA, OrfI et oriT, la région d~origine de la réplication (oriC) d~E. coli et une région plasmidique dérivée d~E. coli dans laquelle l~ADN codant la région terminale N de .beta.-lactamase, produit de l~expression d~un gène de résistance à l~ampicilline (ampR), a été supprimé et dont le nombre de copies moyen est de 6 à 30.

Affiliated with

Also associated with

Rating

Novel shuttle vector does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Novel shuttle vector, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Novel shuttle vector will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1584886