Novel specific arabinose transporter from the yeast pichia...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/81 (2006.01) C07K 14/39 (2006.01) C12P 7/06 (2006.01)

Patent

CA 2673310

The invention relates to a polypeptide featuring a novel specific arabinose transporter function as well as nucleic acids coding therefor. The invention further relates to host cells, particularly modified yeast strains which contain the coding nucleic acids and express the polypeptide and functionally integrate the same into the plasma membrane, thus being able to absorb L-arabinose. When using modified host cells expressing additional proteins of the arabinose metabolic pathway, arabinose can be fermented, especially into ethanol, by means of said cells. The invention is thus significant, among other things, in connection with the production of biochemicals from biomass, e.g. bioethanol.

L'invention concerne un polypeptide présentant une nouvelle fonction de transporteur d'arabinose spécifique, ainsi que des acides nucléiques codant ce polypeptide. Cette invention concerne également des cellules hôtes, en particulier des souches de levure modifiées qui comportent les acides nucléiques codant ledit polypeptide et qui expriment ce polypeptide et l'intègrent fonctionnellement à la membrane plasmatique de manière à pouvoir absorber le L-arabinose. Lors de l'utilisation de cellules hôtes modifiées exprimant d'autres protéines de la voie métabolique de l'arabinose, ce dernier peut être fermenté par ces cellules, en particulier pour produire de l'éthanol. Ainsi, cette invention revêt une importance significative, entre autres, dans le cadre de la production de substances biochimiques à partir d'une biomasse telle que le bioéthanol.

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