Novel synthetic expression vehicle

Details Novel synthetic expression vehicle Novel synthetic expression vehicle

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01)

Patent

CA 2744114

An expression vehicle comprising an isolated nucleic acid as shown in Seq ID No. 1 comprising of a synthetic hybrid promoter wherein the hybrid promoter comprises of an inducible arabinose promoter derived from E.coli and a synthetic stretch of 8-35 nucleotides from pHO regulon introduced in the region between - 55 to -10 and ribosome binding site from the transcription initiation site.

Linvention concerne un véhicule dexpression qui comprend un acide nucléique isolé tel que représenté dans la SEQ ID No. 1, qui comprend un promoteur hybride synthétique. Selon linvention, le promoteur hybride synthétique comprend un promoteur arabinose inductible dérivé dE. coli et une suite synthétique de 8 à 35 nucléotides du régulon pHO introduits dans la région comprise entre -55 et -10 et le site de liaison au ribosome à partir du site dinitiation de la transcription.

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